KMID : 0525720120170010014
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Journal of Chitin and Chitosan 2012 Volume.17 No. 1 p.14 ~ p.18
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Optimization of the Culture Condition for the Production of Chitosanase by Bacillus cereus D-11 and the Chitooligosaccharide Hydrolysis Pattern of Its Enzyme Preparation
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Gao Xing-Ai
Ju Wan-Taek Zhang Yong Feng Jin Rong De Liu Si Yan Park Ro-Dong
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Abstract
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A chitosanase-producing bacterium, Bacillus cereus D-11, was isolated from an environmental soil sample. The optimal culture condition for chitosanase production by B. cereus D-11 was 3 days of cultivation at 30 o C in a medium composed of 0.5% inoculation concentration (1.4¡¿10 8 CFU/mL), 0.7% colloidal chitosan, 1% yeast extract and 1% NaCl at initial pH 7.0. The highest activity achieved was 7.8 U/mL. The crude enzyme preparation from Bacillus cereus D-11 degraded the chitooligomers pentamer, hexamer, and heptamer into (GlcN)2-4, but not degraded the chitooligomers less than (GlcN)4. These results indicate that B. cereus D-11 chitosanase cleaves the oligomeric chains in the endo-splitting manner and the catalytic domain of D-11 chitosanase recognizes and needs at least 5 glucosamine units for hydrolytic catalysis of the glycosidic linkages.
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KEYWORD
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Chitosanase, Bacillus cereus D-11, Optimization, Hydrolysis products
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